Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2022 , Vol 26 , Issue 4
Chemical composition and in vitro mutagenic, antioxidant and anti-inflammatory activities of Lavandula angustifolia Mill. essential oil from Turkey
Pınar Sinem OMURTAG ÖZGEN1,Sevde Nur BİLTEKİN2,Tuğba İDUĞ4,Caglar MACIT5,İrem AYRAN6,Sadiye Ayse ÇELİK6,Şükran KÜLTÜR7,Yüksel KAN6,Gülden Zehra OMURTAG8
1Department of Analytical Chemistry, School of Pharmacy, Istanbul Medipol University, Istanbul, Turkey
2Department of Pharmaceutical Microbiology, School of Pharmacy, Istanbul Medipol University, Istanbul, Turkey
3Institute of Sciences, Istanbul University, Istanbul, Turkey
4Department of Pharmacognosy, School of Pharmacy, Istanbul Medipol University, Istanbul, Turkey
5Department of Pharmacology, School of Pharmacy, Istanbul Medipol University, Istanbul, Turkey
6Department of Medicinal Plants, Faculty of Agriculture, Selcuk University, Konya, Turkey
7Department of Pharmaceutical Botany, Faculty of Pharmacy, Istanbul University, Istanbul, Turkey
8Department of Toxicology, School of Pharmacy, Istanbul Medipol University, Istanbul, Turkey
DOI : 10.29228/jrp.175 The essential oil obtained from Lavandula angustifolia Mill. (Lavander) has many pharmacological effects, including antioxidant, antifungal, antibacterial and carminative effects. Lavandula angustifolia essential oil (LEO) contains monoterpene compounds, mainly linalool and linalyl acetate and various phenolic acids such as caffeic acid, vanillic acid, ferulic acid and chlorogenic acid. The hydrodistillation of the aerial parts of L. angustifolia produced a pale yellow oil. Gas chromatography-Gas chromatography/Massspectrometry (GC-GC/MS) results showed that LEO contains monoterpene alcohols that primarily include linalool (35.91%), 4-Terpineol (6.10%), α-Terpineol (4.49%) and lavandulol (2.49%). As a result of the cytotoxicity analysis of LEO, the IC50 value was found to be 0.372 mg/mL. LEO was found to be non-mutagenic against the bacterial test strain Salmonella typhimurium TA 98 with S9 fraction while the highest concentration (9.58 mg/mL) was found to be either mutagenic or cytotoxic against TA 98 without S9 fraction. The essential oil’s antioxidant capacity was found 6.522±0.069 mg/mL while the total phenolic content was 1.22±0.04 mg GAE/g extract (Gallic acid equivalent/g extract). Anti-inflammatory activity of LEO was 1.238± 0.026 mg/mL while that of indomethacine was 0.022±0.003 mg/mL. In conclusion, when the results are evaluated in terms of in vitro biological activities, LEO can be considered as a potential herbal product with active compounds that should be examined in future studies. Keywords : Lavandula angustifolia; essential oil; mutagenity; antioxidant activity; cytotoxicity
Marmara University