Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2022 , Vol 26 , Issue 4
Methanolic extract of Eryngium creticum Lam leaves, flowers and roots: Quantification and qualification of phenolic contents, antitumor effect and antioxidant capacity
1Department of Clinical Pharmacy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan
2Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal
3Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan
4Department of Chemistry, Faculty of Science, Jerash University, Jerash, Jordan
5Department of Pharmacology and Biomedical Sciences, Faculty of Pharmacy, University of Petra, Amman, Jordan
DOI : 10.29228/jrp.174 Natural products are potential sources of prospective antitumor agents. The phenolic and flavonoid contents of leaves, flowers, and roots of Eryngium creticum were quantified using liquid chromatography-tandem mass spectrometry (LC-ESI-MS/MS). The antioxidant capacity and antitumor activity of the three extracts were tested on MCF-7 and Hep2. The average total phenolic contents of E. creticum leaves, flowers, and roots were 12.43 ± 0.24, 10.09 ± 0.20, and 4.78 ± 0.10 mg of GAE/g of dw, respectively. The average total flavonoids were 4.77 ± 0.10, 6.23 ± 0.12, and 5.62 ± 0.12 mg of QE/g of dw, respectively. The extracts showed considerable DPPH˙ scavenger activity with average IC50 values of 1.41 ± 0.06, 2.70 ± 0.06, and 3.03 ± 0.05 μg/mL, respectively. The average IC50 values of scavenging capacity of NO˙ radicals were 0.11 ± 0.03, 0.69 ± 0.04, and 0.44 ± 0.01 μg/mL, respectively. The average IC50 values in MCF-7 were 1.25 ± 0.50, 3.75 ± 0.48, and 1.88 ± 0.28 mg/mL, respectively. In Hep2, the average IC50 values were 1.16 ± 0.58, 3.75 ± 0.79, and 1.31 ± 0.47 μg/mL, respectively. Phenolic content varied between the different extracts; leaves showed the highest levels of gallic acid (3.60 μg/mL), querciti-3-O-galactoside (0.82 μg/mL), cyanin chloride (1.52 μg/mL), and rosmarinic acid (4.65 μg/mL). Roots showed the lowest quantities of the measured phenolics, except for chlorogenic acid (6.00 μg/mL) and rosmarinic acid (1.86 μg/mL). The three extracts of E. creticum possess in vitro antitumor activities, which could be utilized as an adjunct with chemotherapy. Keywords : Eryngium; plant extract; phenolic compounds; cytotoxicity; breast cancer; antitumor agents
Marmara University