¹Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia
²Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia
³Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia
⁴Postgraduate Program, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia DOI : 10.29228/jrp.154 Doxorubicin is an important drug, especially in the treatment of cancer. But the effectiveness of its use is inseparable from its side effects, such as nephrotoxicity. This study aimed to examine the protective effect in doxorubicin-induced rats of Extract Ethanol of Lansium parasiticum leaves (EELP). The antioxidant activity of EELP was identified using the DPPH method and traced the total phenol content using the Folin-Ciocalteu method and total flavonoids using the colorimetry method. Oxidative stress and renal injury induced in doxorubicin treated rats were proved by the significant elevation of urea and creatinine and alteration in oxidative stress markers [MDA and GSH levels]. Histopathology of organs was examined under a microscope to see the damage that occurs in the tissue. The measurement results of antioxidant activity showed that EELP had a strong activity with an IC50 value of 14.8±0.7 μg/mL, 107.5±0.8 μg GAEs/mg extract for phenol content, and 33.6±0.3 μg quercetin/mg extract for flavonoid content. EELP was able to reduce MDA, urea, creatinine and increase GSH level. Observation of kidney tissues revealed a protective effect of EELP. This was characterized by a reduction in the type of damage that occurs in the kidney tissue of doxorubicin-induced rats. This study suggests that EELP through its antioxidant properties has a protective effect against doxorubicin-induced nephrotoxicity. Keywords : Nephrotoxicity; Doxorubicin; Lansium parasiticum; Oxidative stress