Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2022 , Vol 26 , Issue 3
The effect of Cotinus coggygria L. ethanol extract in the treatment of burn wounds
Buşra ERTAŞ1,Betül OKUYAN2,Ali ŞEN3,Feriha ERCAN4,Hüseyin ÖNEL1,Fatih GÖĞER5,Göksel ŞENER6
1Department of Pharmacology, School of Pharmacy, Marmara University, İstanbul, Turkey
2Department of Clinical Pharmacy, School of Pharmacy, Marmara University, İstanbul, Turkey
3Department of Pharmacognosy, School of Pharmacy, Marmara University, İstanbul, Turkey
4Department of Histology and Embryology, School of Medicine, Marmara University, İstanbul, Turkey
5Department of Pharmacognosy, School of Pharmacy, Anadolu University, Eskişehir, Turkey
6Department of Pharmacology, School of Pharmacy, Fenerbahçe University, İstanbul, Turkey
DOI : 10.29228/jrp.153 The overall aim of the present research is to evaluate for the first time the curative effect of Cotinus coggygria leaves on burn injury in an experimental burn model along with its anti-inflammatory and antioxidant activity potential. Also, phenolic compounds of C. coggygria were characterised by LC-MS/MS. Wistar albino rats weighing 200- 250 g were exposed to 90°C bath for 10 s to induce burn injury, involving 30% of the total body surface area. In the treatment groups, 5% C. coggygria ethanol extract was applied topically as a cream immediately after the burn. Blood and skin tissue samples were taken after decapitation at the 4th and 48th hours following the burn procedure. Interleukin 1-β (IL-1β) and tumour necrosis factor (TNF-α) were determined in serum samples, and hydroxyproline, prostoglandin E2 (PGE2), and myeloperoxidase (MPO) activity and 8-hydroxy-2′-deoxy-guanosine (8-OHdG) levels were determined in skin tissue samples. Increased levels of serum cytokines were decreased with C. coggygria treatment in both periods. MPO activity, prostaglandine (PGE2), and 8-OhdG levels increased, while hydroxyproline levels decreased due to burn damage. On the other hand, these parameters were returned to its normal levels with C. coggygria treatment. In addition, the tissue histology of animals treated with C. coggygria showed a complete epithelialization with increased collagenation. As a result, C. coggygria may be an alternative treatment approach for burns-induced skin damage and wounds. Keywords : Antioxidant activity; anti-inflammatory activity; burn wound healing activity; Cotinus coggygria; phenolic compounds
Marmara University