Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2022 , Vol 26 , Issue 3
Time and Concentration Dependent Effects of Different Solvents on Proliferation of K562, HL60, HCT-116 and H929 Cell Lines
Asli KOC1,Arzu Zeynep KARABAY1,Tulin OZKAN2,Zeliha BUYUKBINGOL1,Fugen AKTAN1
1Department of BIOCHEMISTRY, Faculty of PHARMACY, ANKARA University, Ankara, Turkey
2Department of MEDICAL BIOLOGY, Faculty of MEDICINE, ANKARA University, Ankara, Turkey
DOI : 10.29228/jrp.146 Many organic solvents are used in cell culture models to dissolve polar and non-polar chemical compounds and drugs to test their activities. However, the solvents themselves can have cytotoxic effects on cells. The degree of cytotoxic effect may vary according to the solvent used. The aim of this study is to determine the time and concentration dependent effects of methanol, ethanol, dimethyl sulfoxide (DMSO), ethyl acetate and acetone on colorectal cancer (HCT-116), chronic myeloid leukemia (K562), multiple myeloma (H929) and acute promyelocytic leukemia (HL60) cell lines. According to our results, DMSO is the most toxic solvent and ethyl acetate and methanol are least toxic solvents in all cells. IC50 value of Acetone is greater than 10% in K562, HL60 and HCT-116 cells in all tested incubation times but in H929 cells IC50 value of acetone is decreased over time. In conclusion, since most of these solvents are currently being used for treatment of cells with different drugs and compounds in biochemical toxicology studies, it is essentially important to reveal their biochemical and toxic effects to determine the optimum concentration to be used. Keywords : DMSO; Methanol; Acetone; Ethanol; cytotoxicity; cell culture; MTT; cancer
Marmara University