Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2022 , Vol 26 , Issue 2
Platanus orientalis (Plane Tree) Extract Protects Against Hyperoxaluria Induced Kidney Damage
Betül AYAZ ADAKUL1,Ali ŞEN2,T. Emre ŞENER3,Ömer ERDOĞAN4,Özge ÇEVİK4,Pınar EKER5,Şule ÇETİNEL6,Furkan BÖLÜKBAŞI6,İsmail ŞENKARDEŞ7,Büşra ERTAŞ1,Göksel ŞENER8
1Department of Pharmacology, Faculty of Pharmacy, Marmara University, Istanbul, Turkey
2Department of Pharmacognosy, Faculty of Pharmacy, Marmara University,Istanbul, Turkey
3Department of Urology, Faculty of Medicine, Marmara University, Istanbul, Turkey
4Department of Biochemistry, Faculty of Medicine, Aydin Adnan Menderes University, Aydin, Turkey
5Department of Biochemistry, Umraniye Research and Training Hospital, Health Sciences University, Istanbul, Turkey
6Department of Histology and Embryology, Faculty of Medicine, Marmara University, Istanbul, Turkey
7Department of Pharmaceutical Botany, Faculty of Pharmacy, Marmara University, Istanbul, Turkey
8Vocational School of Health Services, Fenerbahce University, Istanbul, Turkey DOI : 10.29228/jrp.129 The aim of this study is to determine whether Platanus orientalis (PO) which has anti-inflammatory, antioxidant and diuretic properties and used in the treatment of kidney stones as traditional folk medicine, will reduce or prevent the stone formation in the urinary system. To simulate the urolithiasis model 0.75% ethylene glycol (EG) and 1% ammonium chloride (AC) were applied to Sprague-Dawley rats. The rats were divided into four groups (n=8). The control group was given standard drinking water for 5 weeks. EG group received 0.75% EG in their drinking water containing 0.75% EG and 1% AC. PO extract (100 mg/kg) was given orally for 5 weeks to the preventive group and for last 2 weeks to the therapeutic group, respectively. At the end of experiment, 24-hour urine and kidney samples were obtained. In urine samples, calcium and citrate levels were decreased and oxalate level was increased in the EG group. In kidney samples myeloperoxidase, caspase-3, N-acetyl-β-glycosaminidase (NAG) activities, malondialdehyde (MDA), 8-hydroxy-2-deoxyguanosine (8-OHdG), tumor necrosis factor-α and interleukin-1β levels were increased while superoxide dismutase activity and glutathione levels were decreased in the EG group. These biochemical parameters returned to control levels in both PO treatment groups. Histological findings also correlate with these results. Our findings are suggested that PO treatments is effective in both preventive and therapeutic groups. Keywords : Platanus orientalis; kidney stone; urolithiasis; rat
Marmara University