Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2024 , Vol 28 , Issue 3
BBD-driven optimization of an RP-HPLC method for simultaneous analysis of two major ısoflavone aglycones in Tofu
Florentinus Dika Octa RISWANTO1,Dina Christin Ayuning PUTRI2,Michael Raharja GANI1
1Division of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Sanata Dharma, Yogyakarta, Indonesia
2Division of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Sanata Dharma, Yogyakarta, Indonesia
DOI : 10.29228/jrp.731 Tofu, one of soy food products widely consumed in Indonesia, has been reported as a source of natural protein. It is important to evaluate the contents of genistein and daidzein to provide information according to the nutrient information on soy food products. A reversed-phase high performance liquid chromatography (RP-HPLC) was developed in this study in order to analyze genistein and daidzein content in tofu samples simultaneously. Employing a Box-Behnken design (BBD) for designing the experiment, the response surface methodology (RSM) was then applied to optimize chromatographic conditions including methanol composition, flowrate, and column temperature. Separation response such as retention time, resolution, and tailing factor were evaluated to build an optimization model followed by analyzing the desirability. It was found that the model predicted a composite desirability of 0.9778 can be obtained by applying the methanol composition of 60%, flowrate of 0.80 mL.min-1, and column temperature of 50ºC. The optimized HPLC conditions met the acceptance criteria for retention time and area deviations, resolution, tailing factor, and theoretical plates number. Keywords : daidzein; genistein; experimental design; tofu
Marmara University