Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2021 , Vol 25 , Issue 5
HPTLC-DPPH• and HPTLC-tyrosinase methods for hot water-soluble contents of kumquat, limequat and Mexican lime fruit powders
Hale Gamze AĞALAR1,Burak TEMİZ1
1Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Tepebaşı, 26470, Eskişehir, Turkey DOI : 10.29228/jrp.48 In the present study, small Citrus fruits which are cultivated in our country and have an economic value were chosen as research materials. It was aimed to do fingerprint analysis of the fruits by HPTLC method. DPPH• scavenging and antityrosinase potentials were determined in vitro. Extracts showed noticable activities, but these effects were not comparable with positive controls. HPTLC-bioautographic assays and Mass Spectrometry analysis were employed to identify bioactive compound in the extracts. Phloretin-di-C-glucoside was tentatively identified as DPPH• scavenging and antityrosinase active compound in kumquat and limequat extracts. Keywords : Kumquat; limequat; Mexican lime; HPTLC-DPPH• assay; HPTLC-tyrosinase assay; phloretin-di-C-glucoside
Marmara University