Editor-in-Chief İlkay Küçükgüzel Associate Editor Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Quarterly (January-April-July-October) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2019 , Vol 23 , Issue 2
The development of forced degradation studies for the determination of daptomycin in urine at various conditions by using RP-LC method
Bediha AKMEŞE1,Nurgül K. BAKIRHAN1,Tuğçe GÖVER2,Sibel A. ÖZKAN3
1Department of Chemistry, Faculty of Art and Science, Hitit University, Çorum, Turkey
2Department of Analytical Chemistry, Faculty of Pharmacy, Selçuk University, Konya, Turkey
3Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, Ankara, Turkey
DOI : 10.12991/jrp.2019.122 Daptomycin is known as the first lipopeptide antibiotic shows activity against gram-positive bacteria. A fast, simple and repeatabile RP-LC method has been developed for the determination of daptomycin in mobile phase solution and human urine. This drug was exposed to various stress conditions such as hydrolytic, oxidative, thermal and photolytic and the stressed samples were detected by the proposed method. Daptomycin was well separated on a X-Terra RP-18 column using the mobile phase consisting of a mixture of acetonitrile:water (45:55 v/v) at a flow rate of 1 mL min-1. The system operates at 222 nm for daptomycin and 319 nm and tinidazole was used as internal standard under these conditions. The linearity of daptomycin was obtained between 0.50 and 12 μg mL-1 concentration range. Limits of detection was determined as 0.1176 and 0.1412 μg mL-1 and the limits of quantification was obtained as 0.3563 and 0.4277 μg mL-1 in the mobile phase and urine, respectively. Hydrochloric acid, sodium hydroxide, hydrogen peroxide affected response of daptomycin under stress degradation conditions. Keywords : Drug; daptomycin; degradation conditions; high performance liquid chromatography
Marmara University